Using four-wave mixing in a hot atomic vapor, we generate a pair of entangled twin beams in the microsecond pulsed regime near the D1 line of 85Rb, making it compatible with commonly used quantum memory techniques. The beams are generated in the bright and vacuum-squeezed regimes, requiring two separate methods of analysis, without and with local oscillators, respectively. We report a noise reduction of up to 3.8 ± 0.2 dB below the standard quantum limit in the pulsed regime and a level of entanglement that violates an Einstein–Podolsky–Rosen inequality.